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30.09.1999 | 11:17 AM
Morphometric Dual-Energy X-Ray Absorptiometry of the Spine: Report of a Large Series and Correlation with Axial Bone Mineral Density.
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We studied vertebral morphometry and its relation to bone mineral density (BMD) in normal Brazilian women (n = 605). All women (age 22–97 years) were ambulatory and healthy. A lateral spine scan was done for morphometric X-ray absorptiometry using an imaging densitometer. In 429 of these women, BMD of the spine and proximal femur also […]

30.08.1999 | 11:23 AM
Development of Compensatory Thermogenesis in Response to Overfeeding in Hypothyroid Rats.
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Intact or surgically thyroidectomized (Tx) adult male Wistar rats, weighing 150-200 g, were fed a standard chow diet (approximately 1.8 Cal/g) or a high calorie (approximately 3.8 Cal/g) diet (cafeteria diet) for up to 30 days. Daily energy intake was about 5-fold higher in the rats fed the cafeteria diet regardless of their thyroid status. […]

30.01.1999 | 11:29 AM
3,5,3 -Triiodothyronine actively stimulates UCP in brown fat under minimal sympathetic activity.
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To investigate the role of type II 5′-deiodinase (5’D-II) in the expression of mitochondrial uncoupling protein (UCP) in brown adipose tissue (BAT), we injected intact male rats with reverse (r) 3,5,3′-triiodothyronine (T3; 100 micrograms. 100 g body wt-1. day-1), an inhibitor of 5’D-II, for 2-5 days. UCP decreased by approximately 20% in rats kept at […]

20.02.1998 | 11:35 AM
Thyroxine 5 -Deiodination Mediates Norepinephrine- Induced Lipogenesis in Dispersed Brown Adipocytes.
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In euthyroid rats, maximal sympathetic nervous system stimulation (e.g. during cold exposure) results in a 3- to 4-fold increase in brown adipose tissue lipogenesis, a response that is blunted in hypothyroid rats. To further investigate this phenomenon, the role of local type II 5′-deiodinase (5′-DII) was studied in freshly isolated brown adipocytes. In a typical […]

04.08.1996 | 9:54 AM
Effects of Hypothyroidism on Brown Adipose Tissue Adenylyl Cyclase Activity.
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Hypothyroidism profoundly reduces the capacity of brown adipose tissue (BAT) to generate CAMP in response to adrenergic stimulation. Evidence obtained with isolated brown adipocytes suggests a postre- ceptor defect that offsets the hypothyroidism-induced increase in /.%- adrenergic receptors. The goal of the present studies was to identify the defect in the CAMP generation pathway for […]

04.01.1996 | 10:03 AM
The Use of Recombinant by Chinese Hamster Ovary Cells for the Preparation of Immunoassay Reagents.
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Recombinant human TSH (ret-hTSH; Thyrogen, lot M-17073) obtained from transformed Chinese hamster ovary cells was tested for both radioiodination and preparation of a secondary standard used in RIA and immunoradiometric assay (IRMA) for routine clinical inves- tigation. Results were compared to those obtained with high quality pituitary TSH (pit-hTSH; Dr. P. Tojesen, Oslo, Norway; and […]

04.09.1994 | 10:18 AM
Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue.
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The calcium- and phospholipid-dependent protein kinase-C (PKC) is a critical enzyme of cellular signal transduction. In this report we studied calcium-dependent total PKC activity in eight adrenocortical carcinomas (group 1), nine adrenocortical adenomas (group 2), six hyperplasias (group 3), and five human normal adrenal tissues (group 4). The PKC activity assay was based on phosphorylation […]